Part:BBa_K5143006

fwYellow, chromoprotein optimized for expression in Saccharomyces cerevisiae

Description

fwYellow is a chromoprotein that was synthetized to monitor biological processes, like fluorescent proteins, but without the need to use devices to detect the protein. Chromoproteins absorb visible light so that they are visible in the ambient light unlike fluorescent proteins. fwYellow was first synthetized by the iGEM team Uppsala in 2013 (BBa_K1033910). The excitation wavelength is 520nm and the emission wavelength is 540nm. Here, the fwYellow chromoprotein was transformed into Saccharomyces cerevisiae cocultured with the cellulose-producing bacteria Komagataeibacter rhaeticus in order to make the cellulose yellow. The coloured cellulose, that should also be sticky thanks to a bioglue (BBa_K5143022) would then trap insects that are specifically attracted to yellow.

Figure 1: fwYellow gene

Figure 2: Representation of fwYellow binded to bacterial cellulose

Construction

The fwYellow gene was synthesized and its nucleotide sequence optimized for synthesis and expression in Saccharomyces cerevisiae. We used the fwYellow fused to a yeast secretion signal, the alpha-factor (BBa_K5143009) in 5' and to a cellulose binding domain (BBa_K5143007) in 3'. See the composite part here : BBa_K5143023

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 427
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 427
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 427
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 427
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 427
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. iGEM13_Uppsala
2. Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering 12, 8 (2018).